Votre recherche pour: Merck Millipore (Novagen)

Référence Produit: (69752-3)

Fournisseur: Merck Millipore (Novagen)

Description: The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

UOM: 1 * 10 µG

FDS (Fiche de données de sécurité) *Promo

Fournisseur: Merck Millipore (Novagen)

Description: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Référence Produit: (69078-3)

Fournisseur: Merck Millipore (Novagen)

Description: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

UOM: 1 * 1 Pce

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: Les échelles Perfect DNA™ contiennent des jeux d'espèces d'DNA de taille pratique et aisément mémorisable pour l'analyse de gel d'agarose. Les marqueurs ont des intensités de bande uniformes et couvrent une large gamme de tailles d'DNA.

Fournisseur: Merck Millipore (Novagen)

Description: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Fournisseur: Merck Millipore (Novagen)

Description: Pellet Paint® NF Co-Precipitant est un support coloré non fluorescent compatible avec le séquençage fluorescent. Il facilite l'élimination rapide des terminateurs BigDye® pendant la précipitation à l'alcool des produits de réaction de séquençage. Ces produits peuvent être rapidement précipités, avec une durée de centrifugation de 10 minutes. Ce support aisément visualisable fournit une confirmation simple de la précipitation. Les produits de réaction de séquençage sont rassemblés pour obtenir des culots, les terminateurs colorés restant dans le surnageant avec les protocoles de précipitation standard d'Applied Biosystems. La re-suspension des produits de réaction de séquençage dans du formamide désionisé peut être confirmée en vérifiant la dissolution du culot du support.

Fournisseur: Merck Millipore (Novagen)

Description: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Fournisseur: Merck Millipore (Novagen)

Description: L'ADN polymérase KOD HiFi est une enzyme de correction unique, isolé de l'hyperthermophile <i>Thermococcus kodakaraensis</i> KOD1, qui possède une processivité et une fidélité supérieures permettant une amplification PCR plus rapide et plus précise que les enzymes conventionnels.

Référence Produit: (71840-3)

Fournisseur: Merck Millipore (Novagen)

Description: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

*Promo

Référence Produit: (71978-3)

Fournisseur: Merck Millipore (Novagen)

Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

*Promo

Référence Produit: (69831-3)

Fournisseur: Merck Millipore (Novagen)

Description: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

UOM: 1 * 1 SET

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.

Fournisseur: Merck Millipore (Novagen)

Description: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into <i>E.coli</i> is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Référence Produit: (69959-3)

Fournisseur: Merck Millipore (Novagen)

Description: The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

UOM: 1 * 1 Pce

*Promo