Votre recherche pour: Merck Millipore (Novagen)

Référence Produit: (71840-3)

Fournisseur: Merck Millipore (Novagen)

Description: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: L'ADN polymérase KOD HiFi est une enzyme de correction unique, isolé de l'hyperthermophile <i>Thermococcus kodakaraensis</i> KOD1, qui possède une processivité et une fidélité supérieures permettant une amplification PCR plus rapide et plus précise que les enzymes conventionnels.

Référence Produit: (71978-3)

Fournisseur: Merck Millipore (Novagen)

Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: Les échelles Perfect DNA™ contiennent des jeux d'espèces d'DNA de taille pratique et aisément mémorisable pour l'analyse de gel d'agarose. Les marqueurs ont des intensités de bande uniformes et couvrent une large gamme de tailles d'DNA.

Référence Produit: (69831-3)

Fournisseur: Merck Millipore (Novagen)

Description: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

UOM: 1 * 1 SET

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Fournisseur: Merck Millipore (Novagen)

Description: Pellet Paint® NF Co-Precipitant est un support coloré non fluorescent compatible avec le séquençage fluorescent. Il facilite l'élimination rapide des terminateurs BigDye® pendant la précipitation à l'alcool des produits de réaction de séquençage. Ces produits peuvent être rapidement précipités, avec une durée de centrifugation de 10 minutes. Ce support aisément visualisable fournit une confirmation simple de la précipitation. Les produits de réaction de séquençage sont rassemblés pour obtenir des culots, les terminateurs colorés restant dans le surnageant avec les protocoles de précipitation standard d'Applied Biosystems. La re-suspension des produits de réaction de séquençage dans du formamide désionisé peut être confirmée en vérifiant la dissolution du culot du support.

Fournisseur: Merck Millipore (Novagen)

Description: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Fournisseur: Merck Millipore (Novagen)

Description: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Fournisseur: Merck Millipore (Novagen)

Description: D-Tube™ dialyser, D-Tube™ electroelution accessory kit

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Référence Produit: (70852-3)

Fournisseur: Merck Millipore (Novagen)

Description: Le kit SpinPrep™ Gel ADN permet l'extraction efficace de fragments d'ADN de 150 bp à >12 000 bp à partir de gels d'agarose. La procédure utilise une solution GelMelt™ pour dissoudre la plaque de gel, avant l'adsorption de l'ADN par une membrane de silice dans un format de colonne de centrifugation. Après une étape de lavage, l'ADN purifié est élué dans du tampon de faible concentration en sels. Chaque colonne de centrifugation peut lier jusqu'à 20 µg d'ADN. Les récupérations sont toujours comprises entre 50 et 90% dans des conditions standard.

UOM: 1 * 100 Tests

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Référence Produit: (70607-3)

Fournisseur: Merck Millipore (Novagen)

Description: Loading Buffer, 4X SDS sample buffer

UOM: 1 * 2 mL

*Promo

Fournisseur: Merck Millipore (Novagen)

Description: Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Référence Produit: (69203-3)

Fournisseur: Merck Millipore (Novagen)

Description: Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

UOM: 1 * 5 mL

*Promo