Votre recherche pour: Thermo Fisher Scientific

Fournisseur: Thermo Fisher Scientific

Description: Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Fournisseur: Thermo Fisher Scientific

Description: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Certificats.

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Certificats.

Référence Produit: (R0641)

Fournisseur: Thermo Fisher Scientific

Description: In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA. RNA loading dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilises RNA.

UOM: 1 * 1 KIT

*Promo

Référence Produit: (SM0271)

Fournisseur: Thermo Fisher Scientific

Description: pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Thermo Fisher Scientific

Description: T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

FDS (Fiche de données de sécurité)

Fournisseur: Thermo Fisher Scientific

Description: AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Référence Produit: (R1181)

Fournisseur: Thermo Fisher Scientific

Description: Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

UOM: 1 * 1 KIT

*Promo

Référence Produit: (FERMR0531)

Fournisseur: Thermo Fisher Scientific

Description: TurboFect transfection reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

UOM: 1 * 1 KIT

FDS (Fiche de données de sécurité) *Promo

Fournisseur: Thermo Fisher Scientific

Description: This nuclease-free water is deionized and 0.22 µM membrane-filtered nuclease-free water

Référence Produit: (FD1194)

Fournisseur: Thermo Fisher Scientific

Description: GG site and cuts best at 37 °C in 5 to 15 minutes using universal FastDigest buffer.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Thermo Fisher Scientific

Description: The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

Fournisseur: Thermo Fisher Scientific

Description: GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.

Référence Produit: (R0242)

Fournisseur: Thermo Fisher Scientific

Description: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.

UOM: 1 * 1 KIT

*Promo