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Votre recherche pour: Réactifs pour l'extraction des protéines

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Image Unavailable-ENZOBMLKI1050300

Fluor-de-Lys® Developer I concentrate

Référence Produit: (ENZOBMLKI1050300)
Fournisseur: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl
Product Image-786-088

Carrier proteins

Fournisseur: G-Biosciences
Description: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Fournisseur: G-Biosciences
Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Certificats.

Product Image-786-128

Detergent removal systems, OrgoSol DetergentOUT™

Fournisseur: G-Biosciences
Description: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Référence Produit: (PIER90051)
Fournisseur: Thermo Fisher Scientific
Description: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
UOM: 1 * 20 µG
Product Image-786-428

Adenine deaminase

Référence Produit: (786-428)
Fournisseur: G-Biosciences
Description: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
UOM: 1 * 0,25 mL
Product Image-PIER84841

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Référence Produit: (PIER84841)
Fournisseur: Thermo Fisher Scientific
Description: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
UOM: 1 * 10 µG
Product Image-PIER20343

Immobilized pepsin, Pierce™

Référence Produit: (PIER20343)
Fournisseur: Thermo Fisher Scientific
Description: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
UOM: 1 * 5 mL
Product Image-PIER88305

Detergent removal spin column kit, HiPPR™

Fournisseur: Thermo Fisher Scientific
Description: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Product Image-PIER20341

Immobilised papain, Pierce™

Référence Produit: (PIER20341)
Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
UOM: 1 * 5 mL
Product Image-PIER87777

Pierce™ Detergent Removal Spin Columns and Resin

Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Product Image-786-791

Immobilised Pepsin

Référence Produit: (786-791)
Fournisseur: G-Biosciences
Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
UOM: 1 * 1 Pce
Product Image-786-790

Immobilised Papain

Référence Produit: (786-790)
Fournisseur: G-Biosciences
Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM: 1 * 1 Pce
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Référence Produit: (PIER20308)
Fournisseur: Thermo Fisher Scientific
Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
UOM: 1 * 1 KIT
Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Référence Produit: (PIER88216)
Fournisseur: Thermo Fisher Scientific
Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
UOM: 1 * 1 Pce
Product Image-116540439.

Lysing matrix Z, MP Biomedicals

Fournisseur: MP Biomedicals
Description: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

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