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Votre recherche pour: Protéines expression

Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labelled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.

Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labelled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.


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TriLink® CleanCap® EPO mRNA (5moU)

Fournisseur: TriLink BioTechnologies
Description: EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko <em>et al.</em> showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.

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TriLink® CleanCap® FLuc mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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TriLink® CleanCap® Cas9 mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.

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TriLink® CleanCap® OVA mRNA

Fournisseur: TriLink BioTechnologies
Description: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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TriLink® CleanCap® M6 Cre mRNA (N1MePsU)

Fournisseur: TriLink BioTechnologies
Description: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyses recombination between two loxP sites.

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TriLink® CleanCap® M6 mCherry mRNA (N1MePsU)

Fournisseur: TriLink BioTechnologies
Description: <p>mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma </em>sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.</p>

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TriLink® CleanCap® mCherry mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma sp.</em> mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

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TriLink® CleanCap® M6 FLuc mRNA (N1MePsU)

Fournisseur: TriLink BioTechnologies
Description: The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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TriLink® CleanCap® M6 Cas9 mRNA (N1MePsU)

Fournisseur: TriLink BioTechnologies
Description: <p>Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes </em>SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.</p>

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TriLink® CleanCap® EGFP mRNA

Fournisseur: TriLink BioTechnologies
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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TriLink® CleanCap® Cre mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites.

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TriLink® CleanCap® Beta gal mRNA (5moU)

Fournisseur: TriLink BioTechnologies
Description: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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TriLink® CleanCap® Cas9 mRNA

Fournisseur: TriLink BioTechnologies
Description: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

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TriLink® CleanCap® Cas9 Nickase mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: <p>Cas9 Nickase mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.</p>

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TriLink® CleanCap® OVA mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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TriLink® CleanCap® Renilla Luc mRNA (5 moU)

Fournisseur: TriLink BioTechnologies
Description: Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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