Votre recherche pour: Thermo Fisher Scientific

Fournisseur: Thermo Fisher Scientific

Description: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

FDS (Fiche de données de sécurité)

Référence Produit: (R0631)

Fournisseur: Thermo Fisher Scientific

Description: Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Thermo Fisher Scientific

Description: Les échelles d'ADN Thermo Scientific™ GeneRuler™ et O'GeneRuler™ sont des mélanges de fragments d'ADN purifiés par chromatographie. La gamme GeneRuler comprend les échelles les plus courantes, comme l'échelle d'ADN 100 bp et 1 kb. Les échelles d'ADN GeneRuler™ sont disponibles dans deux formats : format classique (fourni dans un tampon TE) et format prêt à l'emploi (prémélangé avec un colorant de chargement d'ADN 6X qui contient le bleu de bromophénol et le xylène cyanol FF). Les échelles d'ADN O'GeneRuler™ sont une autre version prête à l'emploi des échelles d'ADN GeneRuler™ qui sont pré-mélangées avec le colorant de chargement d'ADN orange 6X.

Fournisseur: Thermo Fisher Scientific

Description: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Référence Produit: (FD1704)

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Thermo Fisher Scientific

Description: Human ribonuclease inhibitor (from placenta)

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Certificats.

Référence Produit: (R0611)

Fournisseur: Thermo Fisher Scientific

Description: This gel loading dye contains two different dyes bromophenol blue and xylene cyanol FF for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.

UOM: 1 * 1 KIT

Certificats. *Promo

Référence Produit: (FD1194)

Fournisseur: Thermo Fisher Scientific

Description: GG site and cuts best at 37 °C in 5 to 15 minutes using universal FastDigest buffer.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Thermo Fisher Scientific

Description: The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

Fournisseur: Thermo Fisher Scientific

Description: GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.

Référence Produit: (R0242)

Fournisseur: Thermo Fisher Scientific

Description: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.

UOM: 1 * 1 KIT

*Promo

Référence Produit: (EP0072)

Fournisseur: Thermo Fisher Scientific

Description: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>. Native<i> Taq</i> DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

UOM: 1 * 1 KIT

*Promo

Référence Produit: (EL0016)

Fournisseur: Thermo Fisher Scientific

Description: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

UOM: 1 * 1 KIT

Certificats. *Promo

Fournisseur: Thermo Fisher Scientific

Description: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.