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Product Image-PIER28313

Detergent solutions, Surfact-Amps™

Fournisseur: Thermo Fisher Scientific
Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Product Image-554-1986

Plaques SPE, 96 puits, SOLA

Fournisseur: Thermo Fisher Scientific
Description: Les plaques SPE 96 puits sans fritté SOLA™ assurent une meilleure reproductibilité avec des extraits plus propres et plus uniformes. Les produits SOLA™ offrent des performances inégalées par rapport aux produits traditionnels de SPE, d'élimination des phospholipides et de précipitation des protéines.

*Promo

Product Image-PIER87779

Pierce™ Detergent Removal Spin Columns and Resin

Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Image Unavailable-BOSSBS-0092R-CY5.5

Anti-CASP10 Rabbit Polyclonal Antibody (Cy5.5®)

Référence Produit: (BOSSBS-0092R-CY5.5)
Fournisseur: Bioss
Description: Caspases are a family of intracellular proteases that mediate cell death and are the principal effectors of apoptosis. Caspase 10 (Mch4, ICE-LAP4, FLICE2) plays an important role in apoptosis induced by a variety of inducers such as TNF alpha and Anti-Fas antibody. It is a large prodomain caspase classified together with caspases 2, 8, and 9 as a signaling caspase. Four isoforms of caspase 10 (caspase 10a, 10b, 10c, and 10d) having the same prodomain but different mature large and small subdomain, have been described. Caspase 10 contains two death domains (DED) involved in linking to the death effector domain of the adapter protein FADD and recruiting the complex to TNFR1 and Fas. The inactive procaspase 10 is variably expressed in many tissues and cell lines as a cytosolic protein. The mature form of caspase 10 comprises two subunits, p23/p17 (splice isoforms) and p12. Interestingly, a caspase 9- dependent processing of caspase 10 by caspase 6 in cell-free extracts has recently been suggested. Caspase 10 can cleave and activate caspases 3, 4, 6, 7, 8, and 9. This is followed by cleavage of numerous key proteins, including the nuclear protein PARP.
UOM: 1 * 100 µl
Product Image-PIER20230

Immobilised trypsin, TPCK treated, Pierce™

Référence Produit: (PIER20230)
Fournisseur: Thermo Fisher Scientific
Description: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM: 1 * 2 mL
Image Unavailable-BOSSBS-12876R-A350

Anti-POU2AF1 Rabbit Polyclonal Antibody (Alexa Fluor® 350)

Référence Produit: (BOSSBS-12876R-A350)
Fournisseur: Bioss
Description: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-12876R-A488

Anti-POU2AF1 Rabbit Polyclonal Antibody (Alexa Fluor® 488)

Référence Produit: (BOSSBS-12876R-A488)
Fournisseur: Bioss
Description: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-12876R-CY5

Anti-POU2AF1 Rabbit Polyclonal Antibody (Cy5®)

Référence Produit: (BOSSBS-12876R-CY5)
Fournisseur: Bioss
Description: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-12876R-A680

Anti-BOB1 Rabbit Polyclonal Antibody (Alexa Fluor® 680)

Référence Produit: (BOSSBS-12876R-A680)
Fournisseur: Bioss
Description: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-12876R-A750

Anti-BOB1 Rabbit Polyclonal Antibody (Alexa Fluor® 750)

Référence Produit: (BOSSBS-12876R-A750)
Fournisseur: Bioss
Description: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-11027R

Anti-KIF5A Rabbit Polyclonal Antibody

Référence Produit: (BOSSBS-11027R)
Fournisseur: Bioss
Description: NKHC1 is a neuronal-specific component of a multi-subunit “molecular motor” complex that mediates intracellular organelle transport. Mutations in the gene encoding NKHC1 cause autosomal dominant spastic paraplegia 10. NKHC1 has a pan-neuronal distribution in the nervous system. Rat tissue extracts by immunoblot of NKHC1 can produce a doublet only in brain and sciatic nerve tissue. NKHC1 is distributed throughout the central nervous system and is enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC1 is concentrated in the perinuclear region of the cell body. Kinesin superfamily proteins like NKHC1 are the molecular motors conveying cargos along microtubules.
UOM: 1 * 100 µl
Product Image-78840.

Subcellular Protein Fractionation Kit for Cultured Cells

Référence Produit: (78840.)
Fournisseur: Thermo Fisher Scientific
Description: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
UOM: 1 * 1 KIT
Image Unavailable-324715-5

Endoproteinase Lys-C, sequencing grade, Lysobacter enzymogenes

Référence Produit: (324715-5)
Fournisseur: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.
UOM: 1 * 5 µG
Product Image-PIER28340

Detergent sampler Kit, Surfact-Amps™

Référence Produit: (PIER28340)
Fournisseur: Thermo Fisher Scientific
Description: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
UOM: 1 * 1 KIT
Product Image-PIER20343

Immobilized pepsin, Pierce™

Référence Produit: (PIER20343)
Fournisseur: Thermo Fisher Scientific
Description: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
UOM: 1 * 5 mL
Product Image-BE-401

Kit d'expérimentation, propriétés physiques des protéines

Référence Produit: (BE-401)
Fournisseur: G-Biosciences
Description: Le kit des propriétés physiques des protéines est une activité de laboratoire qui permet aux étudiants de se pencher sur les propriétés physiques de différentes protéines. Les étudiants se familiarisent avec la solubilité des protéines et apprennent comment elle est affectée par des paramètres variés, tels que la température, le pH, le sel et la constante diélectrique. Ils assimilent la précipitation des protéines due au pH, à une grande concentration de sel et en présence de solvants organiques, et la dénaturation des protéines résultant d'une température élevée. De plus, le kit démontre dans quelle mesure les non-protéines, comme les détergents, modifient considérablement les propriétés physiques des molécules de protéines, ce qui explique l'importance de ces détergents dans la solubilisation des protéines. Cette activité de laboratoire passe par l'analyse de trois types de protéines pures. Les étudiants modifient ensuite certaines de ces propriétés avec un détergent et réexaminent les propriétés physiques de ces protéines. Ils doivent alors observer comment les propriétés physiques des molécules de protéines peuvent être exploitées pour la purification et la caractérisation des protéines, et appliquer leurs résultats à un échantillon test d'extrait de tissu complexe.
UOM: 1 * 1 KIT
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Le stock de cet article est limité mais peut être disponible dans un entrepôt proche de vous. Merci de vous assurer que vous êtes connecté sur le site afin que le stock disponible soit affiché. Si l'call est toujours affiché et vous avez besoin d'aide, s'il vous plaît appelez-nous au 01 45 14 89 12.
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