Votre recherche pour: Systèmes de liaison et de marquage des protéines

Fournisseur: Biotium

Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

FDS (Fiche de données de sécurité)

Fournisseur: Biotium

Description: A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Fournisseur: Biotium

Description: Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for <i>in vivo</i> imaging.

Référence Produit: (92186.)

Fournisseur: Biotium

Description: Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

UOM: 1 * 0,5 mg

*Promo

Fournisseur: Biotium

Description: Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or <i>in situ</i> hybridization (ISH).

Référence Produit: (ENZOENZ42842L0050)

Fournisseur: ENZO LIFE SCIENCES

Description: Orange 552 dUTP (lyophilised)

UOM: 1 * 50 Nmo

New Product *Promo

Référence Produit: (ENZOENZ42842)

Fournisseur: ENZO LIFE SCIENCES

Description: Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for <i>E. coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.

UOM: 1 * 25 Nmo

New Product *Promo

Référence Produit: (ENZOENZ51032K100)

Fournisseur: ENZO LIFE SCIENCES

Description: TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.

UOM: 1 * 1 KIT

New Product *Promo

Fournisseur: Biotium

Description: Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

Référence Produit: (786-404)

Fournisseur: G-Biosciences

Description: CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

UOM: 1 * 10 mL

*Promo

Fournisseur: G-Biosciences

Description: HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

Fournisseur: Biotium

Description: Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

Fournisseur: Biotium

Description: Phalloidin is a toxin isolated from the deadly <i>Amanita phalloides</i> mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

Fournisseur: Biotium

Description: Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Fournisseur: Biotium

Description: Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

Fournisseur: Biotium

Description: Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.