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Votre recherche pour: Systèmes de liaison et de marquage des protéines

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.


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Image Unavailable-ENZOALX850023KI01

Phosphoserine detection set

Référence Produit: (ENZOALX850023KI01)
Fournisseur: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET

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Image Unavailable-ENZOALX620076M001

ZnAF-2 DA

Référence Produit: (ENZOALX620076M001)
Fournisseur: ENZO LIFE SCIENCES
Description: Fluorescent probe for Zn²⁺. Cell permeable.
UOM: 1 * 1 mg

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Image Unavailable-ENZOENZ42853L0050

Aqua 431 dUTP (lyophilised)

Référence Produit: (ENZOENZ42853L0050)
Fournisseur: ENZO LIFE SCIENCES
Description: Aqua 431 dUTP (lyophilised)
UOM: 1 * 50 Nmo

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Image Unavailable-786-696

Biotin kits, Micro HOOK™

Fournisseur: G-Biosciences
Description: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

Image Unavailable-10057.

Reactive oxygen species indicators dyes

Fournisseur: Biotium
Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

FDS (Fiche de données de sécurité)

Product Image-ENZOENZGEN1110050

Nick Translation DNA Labelling System 2.0

Référence Produit: (ENZOENZGEN1110050)
Fournisseur: ENZO LIFE SCIENCES
Description: The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent <i>in situ</i> hybridisation (FISH) applications.
UOM: 1 * 50 Tests

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Product Image-ENZOENZ42811

Bio-16-dUTP

Référence Produit: (ENZOENZ42811)
Fournisseur: ENZO LIFE SCIENCES
Description: Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for <i>E.coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
UOM: 1 * 50 Nmo

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CF™ azide reactive dyes

Fournisseur: Biotium
Description: CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

Image Unavailable-40058.

CF® Dye dCTP conjugates

Fournisseur: Biotium
Description: CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

Image Unavailable-ENZOENZ53005C100

Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)

Référence Produit: (ENZOENZ53005C100)
Fournisseur: ENZO LIFE SCIENCES
Description: Cell viability dye
UOM: 1 * 100 µl

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Image Unavailable-ENZOENZ53009C200

Organelle-ID™ RGB reagent IV

Référence Produit: (ENZOENZ53009C200)
Fournisseur: ENZO LIFE SCIENCES
Description: Organelle-ID RGB® reagent IV is a mixture of cell-permeable red fluorescent endoplasmic reticulum dye, green fluorescent lysosomal dye, and blue fluorescent nucleic acid dye.
UOM: 1 * 200 µl

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Image Unavailable-ENZOBMLKI1070001

AMC (7-Amino-4-methylcoumarin) standard

Référence Produit: (ENZOBMLKI1070001)
Fournisseur: ENZO LIFE SCIENCES
Description: Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.
UOM: 1 * 1 mL

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Product Image-ROCKPA00-00

Avidin, biotin, protein A/G and streptavidin reagents

Fournisseur: Rockland Immunochemicals
Description: Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

Image Unavailable-APOSBIL3115-100ML

HRP - conjugate stabiliser, SeramunStab® STC

Référence Produit: (APOSBIL3115-100ML)
Fournisseur: Apollo Scientific
Description: Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.
UOM: 1 * 100 mL
Product Image-92416.

Mix-n-Stain™ Cyanine dye antibody labelling kits

Fournisseur: Biotium
Description: Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.

Product Image-786-387

femtoCHROMO™- HRP Substrate for chromogenic detection

Fournisseur: G-Biosciences
Description: A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

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