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Votre recherche pour: Systèmes de liaison et de marquage des protéines

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.


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Product Image-92295.

Antibody labelling kits, Mix-n-Stain™ FITC

Fournisseur: Biotium
Description: Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

Product Image-COBSD5-010

R-Phycoerythrin (RPE)

Fournisseur: Columbia Biosciences
Description: Many applications and instruments were developed specifically for R-phycoerythrin.

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ExoBrite™ True EV Membrane Stains

Fournisseur: Biotium
Description: Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

New Product

Product Image-22029.

Tyramide amplification buffer plus

Référence Produit: (22029.)
Fournisseur: Biotium
Description: An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.
UOM: 1 * 1 Pce
Image Unavailable-92224.

Biotin SE protein labelling kit

Référence Produit: (92224.)
Fournisseur: Biotium
Description: This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
UOM: 1 * 1 KIT
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Dye conjugates dUTP CF®

Fournisseur: Biotium
Description: CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for <i>in situ</i> hybridisation and nucleic acid blotting applications.

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Reactive quencher dyes

Fournisseur: Biotium
Description: Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

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Biotin kits, Micro HOOK™

Fournisseur: G-Biosciences
Description: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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ExoBrite™ CTB EV Staining Kits, Biotium

Fournisseur: Biotium
Description: ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

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Bilirubin conjugate, sodium salt

Fournisseur: Cayman Chemical
Description: Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

Image Unavailable-APOSBIL3113-100ML

HRP-conjugate stabiliser, SeramunStab® STNP

Référence Produit: (APOSBIL3113-100ML)
Fournisseur: Apollo Scientific
Description: Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.
UOM: 1 * 100 mL
Product Image-COBSD4-000

B-Phycoerythrin (BPE)

Référence Produit: (COBSD4-000)
Fournisseur: Columbia Biosciences
Description: Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
UOM: 1 * 1 mg

Certificats.


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Reactive oxygen species indicators dyes

Fournisseur: Biotium
Description: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

FDS (Fiche de données de sécurité)

Product Image-786-546

Calmodulin Resin Kit for CBP purification

Fournisseur: G-Biosciences
Description: The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

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ZnAF-2 DA

Référence Produit: (ENZOALX620076M001)
Fournisseur: ENZO LIFE SCIENCES
Description: Fluorescent probe for Zn²⁺. Cell permeable.
UOM: 1 * 1 mg

New Product


Image Unavailable-ENZOALX850023KI01

Phosphoserine detection set

Référence Produit: (ENZOALX850023KI01)
Fournisseur: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET

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