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Product Image-K1642

Maxima™ First Strand cDNA Synthesis kit

Fournisseur: Thermo Fisher Scientific
Description: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Product Image-M1682

Maxima™ H minus cDNA synthesis master mix, with dsDNase

Fournisseur: Thermo Fisher Scientific
Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Product Image-27-9266-01

Billes RT-PCR, illustra™ Ready-To-Go™

Fournisseur: Cytiva
Description: Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.

Product Image-EP0743

Maxima™ Reverse Transcriptase

Fournisseur: Thermo Fisher Scientific
Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Image Unavailable-71978-3

One step RT-PCR master mix kit

Référence Produit: (71978-3)
Fournisseur: Merck Millipore (Novagen)
Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
UOM: 1 * 1 KIT
Product Image-27-9261-01

First-Strand cDNA Synthesis kit

Référence Produit: (27-9261-01)
Fournisseur: Cytiva
Description: Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for <i>in vitro</i> amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
UOM: 1 * 55 Tests
Product Image-K1621

RevertAid™ First strand cDNA synthesis kit

Fournisseur: Thermo Fisher Scientific
Description: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Product Image-K1682

Maxima™ H minus first strand cDNA synthesis kits

Fournisseur: Thermo Fisher Scientific
Description: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Product Image-733-1098

Kits RT-PCR, Verso™

Fournisseur: Thermo Fisher Scientific
Description: Le système RT-PCR Verso™ associe une enzyme de transcription inverse, des options d'amorce améliorées et des tampons optimisés pour générer une amplification pleine longueur des ADNc et un rendement élevé. Grâce à diverses options pour les réactions RT-PCR à une et deux étapes, Verso™ assure une souplesse et une sensibilité optimales dans un format convivial.

Image Unavailable-ICNA11NTPMX105

dNTP mix, MP Biomedicals

Fournisseur: MP Biomedicals
Description: This dNTP mix with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, DNA sequencing and labeling.

Product Image-EP0452

RevertAid™ H Minus Reverse Transcriptase

Fournisseur: Thermo Fisher Scientific
Description: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Product Image-EP0442

RevertAid™ Reverse Transcriptase

Fournisseur: Thermo Fisher Scientific
Description: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Product Image-27-9264-01

PCR beads, Ready-To-Go™ You-Prime First-Strand Beads

Référence Produit: (27-9264-01)
Fournisseur: Cytiva
Description: Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.
UOM: 1 * 50 Tests

FDS (Fiche de données de sécurité)


Image Unavailable-K1612

First strand cDNA synthesis kit

Référence Produit: (K1612)
Fournisseur: Thermo Fisher Scientific
Description: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
UOM: 1 * 1 KIT
Product Image-K1632

RevertAid™ H minus first strand cDNA synthesis kits

Fournisseur: Thermo Fisher Scientific
Description: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Image Unavailable-K0233

Maxima™ Probe qPCR Master Mix (2X)

Fournisseur: Thermo Fisher Scientific
Description: The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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Le stock de cet article est limité mais peut être disponible dans un entrepôt proche de vous. Merci de vous assurer que vous êtes connecté sur le site afin que le stock disponible soit affiché. Si l'call est toujours affiché et vous avez besoin d'aide, s'il vous plaît appelez-nous au 01 45 14 89 12.
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