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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-PIER23238

Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent

Référence Produit: (PIER23238)
Fournisseur: Thermo Fisher Scientific
Description: The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.
UOM: 1 * 300 mL

FDS (Fiche de données de sécurité) Certificats.


Product Image-OZBIBCA2500

BCA protein assay kit (BCA-PAK)

Référence Produit: (OZBIBCA2500)
Fournisseur: OZ BIOSCIENCES
Description: BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.
UOM: 1 * 1 KIT
Product Image-PIER21930

Solid phase biotinylation kits, EZ-Link™, Pierce™

Fournisseur: Thermo Fisher Scientific
Description: This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

Product Image-PIER21277

Pierce™ Polyhis-Protein Interaction Pull Down Kit

Référence Produit: (PIER21277)
Fournisseur: Thermo Fisher Scientific
Description: The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.
UOM: 1 * 1 KIT
Product Image-PIER23260

Pierce™ GlycoProtein Carbohydrate Estimation Kits

Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.

Product Image-PIER23234

Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Fournisseur: Thermo Fisher Scientific
Description: Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

Product Image-PIER48895

Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™

Référence Produit: (PIER48895)
Fournisseur: Thermo Fisher Scientific
Description: Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
UOM: 1 * 50 mg
Product Image-PIER23215

Compat-Able™ Protein assay preparation reagent set

Référence Produit: (PIER23215)
Fournisseur: Thermo Fisher Scientific
Description: The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 500 mL
Image Unavailable-PIER23239

Coomassie Plus™ protein assay kit, Compat-Able™

Référence Produit: (PIER23239)
Fournisseur: Thermo Fisher Scientific
Description: The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 1 KIT
Product Image-PIER16175

Firefly luciferase flash assay kit, Pierce™

Fournisseur: Thermo Fisher Scientific
Description: The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Image Unavailable-71840-3

Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Référence Produit: (71840-3)
Fournisseur: Merck Millipore (Novagen)
Description: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
UOM: 1 * 125 µl
Product Image-PIER23240

Modified lowry protein assay kit, Pierce™

Référence Produit: (PIER23240)
Fournisseur: Thermo Fisher Scientific
Description: The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.
UOM: 1 * 1 KIT

FDS (Fiche de données de sécurité) Certificats.


Product Image-PIER23222

BCA™ protein assay kits and reagents

Fournisseur: Thermo Fisher Scientific
Description: Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

FDS (Fiche de données de sécurité) Certificats.

Image Unavailable-AATB5539

Portelite™ Rapid Fluorimetric Biotin Quantitation Kit

Référence Produit: (AATB5539)
Fournisseur: AAT BIOQUEST
Description: Optimised to use with CytoCite™ and Qubit™ fluorometers. Portelite™ rapid fluorimetric biotin quantitation kit is specifically optimised to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite™ and Qubit™ fluorometer.
UOM: 1 * 50 Tests

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Image Unavailable-AATB11109

Portelite™ Fluorimetric Protein Quantitation Kit

Référence Produit: (AATB11109)
Fournisseur: AAT BIOQUEST
Description: Optimised for CytoCite™ and Qubit™ fluorometers. The Portelite™ fluorimetric protein quantitation kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.
UOM: 1 * 100 Tests

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Image Unavailable-PIER23290

Quantitative fluorometric peptide assay, Pierce™

Fournisseur: Thermo Fisher Scientific
Description: Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

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