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Bleu brillant de Coomassie® R250 ~50% (teneur en colorant), grade Technique, Sigma-Aldrich®

Fournisseur: SIGMA ALDRICH MICROSCOPY
Description: Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250. Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).

Product Image-18-1002-74

Accessories for Multiphor™ II electrophoresis system

Fournisseur: Cytiva
Description: SDS PAGE et Native PAGE, kit IEF, Pour: Multiphor™ II electrophoresis unit

Product Image-BSENM-1385-100

Anti-Nestin Mouse Monoclonal Antibody [clone: 4D11]

Référence Produit: (BSENM-1385-100)
Fournisseur: Biosensis
Description: Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240kDa. However the real molecular weight is considerably less than this, at 177kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.
UOM: 1 * 100 µl
Product Image-80-6484-70

SDS-PAGE Clean Up Kit

Référence Produit: (80-6484-70)
Fournisseur: Cytiva
Description: SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
UOM: 1 * 1 KIT
Image Unavailable-BNUB0707-100

Anti-TNF Mouse Monoclonal Antibody [clone: TNF706 P/T2]

Fournisseur: Biotium
Description: TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Image Unavailable-70607-3

Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®

Référence Produit: (70607-3)
Fournisseur: Merck Millipore (Novagen)
Description: Loading Buffer, 4X SDS sample buffer
UOM: 1 * 2 mL
Product Image-GENSMM1397-500

Protein standards

Fournisseur: Genscript
Description: GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.

Image Unavailable-BTIUBNUM0707-50

Anti-TNF Mouse Monoclonal Antibody (Purified, BSA-free) [clone: TNF706 P/T2]

Référence Produit: (BTIUBNUM0707-50)
Fournisseur: Biotium
Description: TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
UOM: 1 * 50 µl
Image Unavailable-BSENR-1687-100

Anti-ADCY3 Rabbit Polyclonal Antibody

Référence Produit: (BSENR-1687-100)
Fournisseur: Biosensis
Description: Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons, and our antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture. Adenylate cyclase type III is a large complex molecule of, in the human, 1145 amino acids with a deduced molecular weight of 129kDa. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160kDa in cortex and about 200kDa in olfactory epithelium. The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active.
UOM: 1 * 100 µG
Product Image-786-226

Agarose Sealing Solution

Référence Produit: (786-226)
Fournisseur: G-Biosciences
Description: Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.
UOM: 1 * 50 mL
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Phosphate-citrate Buffer (5X)

Référence Produit: (J62982.AE)
Fournisseur: Thermo Fisher Scientific
Description: Phosphate-Citrate Buffer (5X) is used as a substrate buffer in immunoassay procedures. It is used in SDS PAGE electrophoresis.
UOM: 1 * 100 mL

FDS (Fiche de données de sécurité) Certificats.


Product Image-21002-1

Lumitein™ protein gel stain

Fournisseur: Biotium
Description: Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis.

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Anti-FAS Rabbit Polyclonal Antibody

Fournisseur: ENZO LIFE SCIENCES
Description: Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE.  Fas belongs to the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40.  Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells.  Both Fas and TNFR contain a 70 amino acid cytoplasmic “death domain” responsible for transmitting signals for apoptosis.  Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long forms of FLIP. Insertion of an early transposable element in intron 2 of the Fas gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting the involvement of Fas in clonal deletion of autoreactive T cells.

Product Image-BSENC-1382-50

Anti-MAP2 Protein Chicken Polyclonal Antibody

Référence Produit: (BSENC-1382-50)
Fournisseur: Biosensis
Description: Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
UOM: 1 * 50 µl
Product Image-80-6483-56

Kit 2-D Quant

Référence Produit: (80-6483-56)
Fournisseur: Cytiva
Description: Kit développé pour la détermination de protéines concentrées à partir de gels d'électrophorèse 2-D, SDS-PAGE ou IEF.
UOM: 1 * 1 KIT

Certificats.


Product Image-21531-500UL

Prestained protein markers, Peacock™ Plus

Fournisseur: Biotium
Description: A visible three- colour protein marker for SDS-PAGE or western blotting, with 12 bands ranging from 8 to 245 kDa.

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