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Product Image-786-273

Antibody fab fragmentation kit

Fournisseur: G-Biosciences
Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Product Image-786-077

Protein reducing agents

Fournisseur: G-Biosciences
Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Product Image-786-599

Immobilised Iminobiotin Resin

Référence Produit: (786-599)
Fournisseur: G-Biosciences
Description: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Image Unavailable-ENZOBMLPW1075A0001

Proteasome Purification Kit

Référence Produit: (ENZOBMLPW1075A0001)
Fournisseur: ENZO LIFE SCIENCES
Description: Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.
UOM: 1 * 10 Tests
Product Image-DGA01

Optimizer blueBALLS™

Référence Produit: (DGA01)
Fournisseur: G-Biosciences
Description: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 Pce
Product Image-BB01

Botanical garden blot

Référence Produit: (BB01)
Fournisseur: G-Biosciences
Description: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.
UOM: 1 * 1 Pce
Image Unavailable-ENZOALX850024KI01

Phosphothreonine detection set

Référence Produit: (ENZOALX850024KI01)
Fournisseur: ENZO LIFE SCIENCES
Description: This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
UOM: 1 * 1 SET
Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Référence Produit: (ENZOBMLUW99150001)
Fournisseur: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests
Image Unavailable-ENZOADI900198

HSF1 ELISA Kits

Référence Produit: (ENZOADI900198)
Fournisseur: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 in cell lysates and tissue extracts from human and mouse origins. This is a Sensitive ELISA kit for this key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT
Product Image-786-087

Carrier proteins

Fournisseur: G-Biosciences
Description: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Image Unavailable-ENZOADI900199

[pSer326]HSF1 ELISA Kits

Référence Produit: (ENZOADI900199)
Fournisseur: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 phosphorylated at Ser326 in cell lysates and tissue extracts from human and mouse origins. This is a sensitive ELISA kit for the key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT
Product Image-ML-09

Lysates, bacterial GenLysate™, E.Coli

Référence Produit: (ML-09)
Fournisseur: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
UOM: 1 * 150 µG
Product Image-92076.

CF® Dye BCN

Fournisseur: Biotium
Description: CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.

Image Unavailable-BOSSBS-8376R-A750

Anti-RNF160 Rabbit Polyclonal Antibody (Alexa Fluor® 750)

Référence Produit: (BOSSBS-8376R-A750)
Fournisseur: Bioss
Description: E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesised nascent chains for proteasomal degradation (PubMed:23685075, PubMed:25132172, PubMed:25578875). Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product (By similarity).
UOM: 1 * 100 µl
Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Référence Produit: (ENZOBMLKI1761250)
Fournisseur: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL
Product Image-PL-02

Lysates, yeast GenLysate™

Référence Produit: (PL-02)
Fournisseur: G-Biosciences
Description: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
UOM: 1 * 150 µG
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