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Votre recherche pour: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER20148X

Chemiluminescent EMSA kit, LightShift™

Fournisseur: Thermo Fisher Scientific
Description: LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Product Image-PIER34075

Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Fournisseur: Thermo Fisher Scientific
Description: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

Product Image-PIER34012

1-Step™, Pierce™ Chloronaphthol

Référence Produit: (PIER34012)
Fournisseur: Thermo Fisher Scientific
Description: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.
UOM: 1 * 250 mL
Product Image-AATB26100

Amplite® West ECL HRP Substrate *Femto Sensitivity*

Fournisseur: AAT BIOQUEST
Description: Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

New Product

Image Unavailable-PIER15162

NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Référence Produit: (PIER15162)
Fournisseur: Thermo Fisher Scientific
Description: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
UOM: 1 * 1 KIT
Product Image-PIER34042

Substrate for western blotting, 1-Step™ NBT/BCIP

Référence Produit: (PIER34042)
Fournisseur: Thermo Fisher Scientific
Description: The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.
UOM: 1 * 250 mL
Image Unavailable-786-423

High molecular weight transfer buffer (5X)

Référence Produit: (786-423)
Fournisseur: G-Biosciences
Description: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
UOM: 1 * 1 L
Product Image-PIER34070

Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Référence Produit: (PIER34070)
Fournisseur: Thermo Fisher Scientific
Description: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.
UOM: 1 * 100 mL
Product Image-PIER34000

CN/DAB substrate kit, Pierce™

Référence Produit: (PIER34000)
Fournisseur: Thermo Fisher Scientific
Description: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
UOM: 1 * 1 KIT
Product Image-PIER17075

Biotin random prime DNA labelling kit, North2South™

Référence Produit: (PIER17075)
Fournisseur: Thermo Fisher Scientific
Description: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
UOM: 1 * 1 KIT
Product Image-PIER34002

DAB substrate

Référence Produit: (PIER34002)
Fournisseur: Thermo Fisher Scientific
Description: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
UOM: 1 * 1 KIT
Product Image-PIER21230

Pierce™ Clean-Blot™ IP Detection Kit and Reagents

Fournisseur: Thermo Fisher Scientific
Description: Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

Product Image-PIER15165

HRP conjugates, HisProbe™

Référence Produit: (PIER15165)
Fournisseur: Thermo Fisher Scientific
Description: HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
UOM: 1 * 2 mg
Product Image-PIER31490

Horseradish peroxidase, Pierce™

Référence Produit: (PIER31490)
Fournisseur: Thermo Fisher Scientific
Description: Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
UOM: 1 * 10 mg
Product Image-PIER46641

Western blot enhancer, SuperSignal™, Pierce

Fournisseur: Thermo Fisher Scientific
Description: SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

Product Image-PIER90105

S-Nitrosylation western blot kit, Pierce™

Fournisseur: Thermo Fisher Scientific
Description: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

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