Phénol ≥99.0% pour les biotechnologies pH 4.5
Marques : VWR ChemicalsTotal Evaluation: 0
Moyenne des Notes : 0.0 sur 5
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Danger
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Synonyme(s):
Acide phénique
Une alternative idéale pour le phénol saturé en eau dans de nombreux protocoles. Elle offre l'extrême pureté et les performances du phénol saturé en eau avec une stabilité et une sécurité grandement accrues.
- Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
- Liquid phenols offer a safer, easier alternative to crystalline phenol
- Optimised pH for the purification of RNA
- Long term stability > one year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.
Formule:
C₆H₅OH Poids moléculaire: 94,11 g/mol Point d'ébullition: 182 °C Température de stockage: Température ambiante |
Numéro MDL:
MFCD00002143 Numéro CAS: 108-95-2 EINECS: 203-632-7 UN: 2821 ADR: 6.1,II |
Résultats des tests de spécification
O.D.@ 330 nm | ≤0.2 |
O.D.@ 405 nm | ≤0.1 |
O.D.@ 510 nm | ≤0.1 |
pH (Tampon) @25°C | 4.1 – 4.5 |
Pureté | ≥99 % |
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