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EasySep™ Complete Kits for Human Whole Blood CD34+ Cells

Fournisseur: STEMCELL Technologies
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15086RF 15086
STMC15086RFEA 1710 EUR
STMC15086RF STMC15086
EasySep™ Complete Kits for Human Whole Blood CD34+ Cells
Cell Separation Media
Immunomagnetic positive selection kit.

  • Fast and easy-to-use
  • Up to 98% purity
  • No columns required
  • Can be combined with SepMate™ for consistent, high-throughput sample processing

The Complete Kit for Human Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat.

First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail (15186C) with antibodies recognising CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66b and CD61 surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18066C), which contains an antibody recognising CD34. RosetteSep™ binds unwanted cells to red blood cells (RBCs), forming immunorosettes, which sediment during density gradient centrifugation. The pre-enriched fraction containing CD34+ cells is harvested from the interface between the plasma and density gradient medium. The pre-enriched CD34+ cells are then labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated CD34+ cells are immediately available for downstream applications.

Attention: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier’s restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the ‘Product Restrictions’).
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