Bleu nitré-tétrazolium chlorure (NBT) 90 HPLC_ASSAY_METHOD, Sigma-Aldrich®
Marques : SIGMA ALDRICH MICROSCOPYTotal Evaluation: 0
Moyenne des Notes : 0.0 sur 5
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Warning
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Synonyme(s):
5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride
MQ200
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.
- Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells
- It has been used as a substrate for western blot detection
- It has been used for the staining of histological tissue sections
- Substrate for dehydrogenases and other oxidases
Formule:
C₄₀H₃₀Cl₂N₁₀O₆ Poids moléculaire: 817.65 g/mol Point de fusion: 200 Température de stockage: Refrigerator |
Numéro MDL:
MFCD00012159 Numéro CAS: 298-83-9 EINECS: 206-067-4 |
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Résultats des tests de spécification
Appearance (Colour) | Yellow |
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Water (by Karl Fischer) | ≤4.5 % |
Solvent Content (by GC) | ≤10.0 % |
Nitrogen Corrected for Water and Solvent |
16.4 - 17.9 % |
Purity (HPLC) | ≥90.0 % |
Solubility (Colour) c = 10 mg/ml; Water |
Green-Yellow to Dark Yellow |
Solubility (Turbidity) | Clear |
Recommended Retest Period | 3 Years |
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