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VWR®, TEMPase Hot Start 2× master mix

Marques : VWR Chemicals
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733-1841EA 2010 EUR
733-1841 733-1840 733-2569 733-2582 733-2585
VWR®, TEMPase Hot Start 2× master mix
Réactifs pour acides nucléiques End-point PCR Enzymes and Kits

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.


The blue loading dye in Blue TEMPase Hot Start DNA Polymerase Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.


Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR.


Informations de livraison: TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included in the kit to enable optimisation.

Commander maintenant

SPÉCIFICATIONS

Désignation Blue TEMPase Hot Start DNA Polymerase 2X Master Mix C
1.5 mM MgCl2 2500 reactions
TEMPase Hot Start DNA Polymerase 2X Master Mix C
1.5 mM MgCl2 2500 reactions
Multiplex TEMPase 2X Master Mix
1.5 mM MgCl2 2500 reactions
TEMPase Hot Start DNA Polymerase 2X Master Mix A
1.5 mM MgCl2 2500 reactions
Blue TEMPase Hot Start DNA Polymerase 2X Master Mix A
1.5 mM MgCl2 2500 reactions
Environmentally Preferable
Format Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs
Caractéristiques Direct loading Direct loading
Démarrage à chaud Chemically modified Chemically modified Chemically modified Chemically modified Chemically modified
Concentration 2x 2x 2x 2x 2x
Systèmes 2500 r Mix 2500 2500 r Mix 2500 2500 r Mix 2500 2500 r Mix 2500 2500 r Mix 2500
Taille 50 x 1.25 ml 50 x 1.25 ml 50 x 1.25 ml + 3 x 1.5 ml 50 x 1.25 ml 50 x 1.25 ml
Composition du tampon Based on Combination buffer Based on Combination buffer 25 mM MgCl2 Based on Ammonium buffer Based on Ammonium buffer
Haute fidélité Non Non Non Non Non
Taille de l'amplicon ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard Standard
Activité exonucléase 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A 3'A
Conditions de stockage – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Application Multiplex Multiplex Multiplex Multiplex Multiplex

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