Violet cristallisé solution aqueuse éthanolique colorant primaire pour la coloration de Gram, Sigma-Aldrich®
Marques : MerckTotal Evaluation: 0
Moyenne des Notes : 0.0 sur 5
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Attention
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Synonyme(s):
Violet Base 3, CI 42555, Violet de gentiane, Chlorure de 4-[4,4'-bis(diméthylamino) benzhydrylidène]cyclohexa-2,5-dien-1-llidène]diméthylammonium
The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.
The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Formule:
C₂₅H₃₀ClN₃ Poids moléculaire: 407.99 g/mol Température de stockage: Température ambiante |
Numéro MDL:
MFCD00011750 Numéro CAS: 548-62-9 UN: 1170 ADR: 3 III Indice Merck: 13,04406 |
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