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Violet cristallisé solution aqueuse éthanolique colorant primaire pour la coloration de Gram, Sigma-Aldrich®

Marques : Merck
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Synonyme(s): Violet Base 3, CI 42555, Violet de gentiane, Chlorure de 4-[4,4'-bis(diméthylamino) benzhydrylidène]cyclohexa-2,5-dien-1-llidène]diméthylammonium

1.09218.0500 1.09218.2500
1.09218.0500EA 67.7 EUR
1.09218.0500 1.09218.2500
Violet cristallisé solution aqueuse éthanolique colorant primaire pour la coloration de Gram, Sigma-Aldrich®
Violet cristallisé

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.


The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.

Formule: C₂₅H₃₀ClN₃
Poids moléculaire: 407.99 g/mol
Température de stockage: Température ambiante
Numéro MDL: MFCD00011750
Numéro CAS: 548-62-9
UN: 1170
ADR: 3 III
Indice Merck: 13,04406
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