X-Gal (Bromo-5-chloro-4-indolyl-3-ß-D-galactopyranoside)
Marques : G BiosciencesTotal Evaluation: 0
Moyenne des Notes : 0.0 sur 5
Synonyme(s):
BCIG
X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.
Formule:
C₁₄H₁₅BrClNO₆ Poids moléculaire: 408.63 g/mol |
Numéro MDL:
MFCD00005666 Numéro CAS: 7240-90-6 |
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