Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Specifically recognizes the medium neurofilament subunit NF-M in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. Also recognizes the 100 kDa breakdown fragment of NF-M induced by calpain cleavage. The antibody reacts with NF-M from multiple species and multiple applications.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Neurofilament Medium
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Chicken
Isotype: IgY
Reactivity: Human, Rat, Mouse, Cat, Horse, Chicken