Votre recherche pour: RT-PCR and cDNA Reagents

Fournisseur: ENZO LIFE SCIENCES

Description: AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

New Product

Fournisseur: Quantabio

Description: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

FDS (Fiche de données de sécurité)

Fournisseur: Quantabio

Description: Le kit de PCR en point final qScript™ XLT One-Step est un système pratique et très sensible pour l'amplification des modèles d'ARN jusqu'à 2 kb. La synthèse de l'ADN complémentaire et l'amplification PCR sont réalisées dans le même tube sans l'ouvrir entre les procédures. Ce système a été optimisé pour offrir une efficacité, une sensibilité et une spécificité de PCR en point final optimales.

Certificats.

Fournisseur: AGILENT

Description: Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Fournisseur: Quantabio

Description: Les kits de synthèse d'ADNc qScript™ contiennent de la transcriptase inverse qScript™, un mélange de transcriptase inverse MMLV et de protéine inhibitrice de la ribonucléase, optimisée pour une synthèse sensible et fiable de l'ADNc sur une large plage dynamique d'ARN. Les réactifs de synthèse d'ADNc sont fournis dans différents formats 'supermix' et 'kit' correspondant à des applications spécifiques.

FDS (Fiche de données de sécurité) Certificats.

Fournisseur: ENZO LIFE SCIENCES

Description: AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

New Product

Fournisseur: Quantabio

Description: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Fournisseur: Thermo Fisher Scientific

Description: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

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Fournisseur: Thermo Fisher Scientific

Description: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Référence Produit: (K1612)

Fournisseur: Thermo Fisher Scientific

Description: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

UOM: 1 * 1 KIT

*Promo

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Certificats.

Fournisseur: Thermo Fisher Scientific

Description: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Fournisseur: Thermo Fisher Scientific

Description: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Fournisseur: Thermo Fisher Scientific

Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Fournisseur: Thermo Fisher Scientific

Description: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Fournisseur: Thermo Fisher Scientific

Description: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.