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Frequently used for calibration, the chromatography standards have a known concentration to test separating equipment for performance faults. Analytical equipment error would impact the dependability of retention time, purity, molecular weight, and compound identification conclusions. Improving data quality, the substances allow personnel to plot analyte signal ratios and obtain concentrations from a calibration curve. Chromatography standards verify instruments are suitable for intended ion exchange, gel filtration, and hydrophobic interaction procedures.