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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-1B1301-KIT

VWR® Protein Precipitation Kit

Référence Produit: (1B1301-KIT)
Fournisseur: VWR Chemicals
Description: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
UOM: 1 * 1 KIT
Image Unavailable-D8284-5G

Bicinchoninic acid disodium salt hydrate

Fournisseur: SIGMA ALDRICH MICROSCOPY
Description: Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Product Image-ENZOENZ51023KP050

PROTEOSTAT® protein aggregation assay

Référence Produit: (ENZOENZ51023KP050)
Fournisseur: ENZO LIFE SCIENCES
Description: PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
UOM: 1 * 50 Tests

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Product Image-ABCAAB288095-10

Protein G Spin Antibody Purification Kit

Référence Produit: (ABCAAB288095-10)
Fournisseur: Abcam
Description: Kit helps purify antibodies from serum, ascites and cell culture medium from various species.
UOM: 1 * 10 Tests

Market Source Item This is a MarketSource item. Additional charges may apply

Product Image-ABCAAB283400-100

Human Thioredoxin 1 (Trx1) Inhibitor Screening Kit (Fluorometric)

Référence Produit: (ABCAAB283400-100)
Fournisseur: Abcam
Description: Simplest assay to screen/study/characterize thioredoxin 1 inhibitors.
UOM: 1 * 100 Tests

Market Source Item This is a MarketSource item. Additional charges may apply

Image Unavailable-ENZOENZ51029K100

eFluxx-ID® multidrug resistance assay kit

Fournisseur: ENZO LIFE SCIENCES
Description: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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Product Image-ROCKMB-061-1000

Conjugate stabilisers

Fournisseur: Rockland Immunochemicals
Description: Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.

Product Image-786-573

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Fournisseur: G-Biosciences
Description: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Image Unavailable-33021-T

Thermal shift protein stability kits, GloMelt™

Fournisseur: Biotium
Description: Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

Image Unavailable-TNB-1213-L150

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Référence Produit: (TNB-1213-L150)
Fournisseur: Tonbo Biosciences
Description: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
UOM: 1 * 150 mL
Product Image-786-604

Protein assay kits, RED 660™

Fournisseur: G-Biosciences
Description: RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Image Unavailable-CAYM10005020-96

Protein carbonyl colorimetric assay kit

Référence Produit: (CAYM10005020-96)
Fournisseur: Cayman Chemical
Description: The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
UOM: 1 * 96 Tests
Image Unavailable-ENZOBMLUW1000A0001

SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Référence Produit: (ENZOBMLUW1000A0001)
Fournisseur: ENZO LIFE SCIENCES
Description: The only commercially available kit for isolation and enrichment of SUMOylated proteins.
UOM: 1 * 10 Tests

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Product Image-786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Référence Produit: (786-005)
Fournisseur: G-Biosciences
Description: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
UOM: 1 * 1 KIT
Product Image-CAYM700480-96

Glycogen Assay Kit

Référence Produit: (CAYM700480-96)
Fournisseur: Cayman Chemical
Description: A simple and sensitive tool for assaying glycogen from tissue.
UOM: 1 * 1 Pce
Product Image-ABCAAB283408-100

Human β-Secretase (BACE1) Inhibitor Screening Kit (Fluorometric)

Référence Produit: (ABCAAB283408-100)
Fournisseur: Abcam
Description: Highly Sensitive Assay for screening potential BACE1 inhibitors.
UOM: 1 * 100 Tests

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