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Assay buffers are mixtures of buffers, salts, detergents, protein, preservatives, and colouring agents. These buffers are used in ELISA assays for dilution of standards, samples, and reagents, and for blocking of non-specific interactions. Buffers may be used individually or in combination, Buffers may be used individually or in combination. Blocking buffers are specifically designed to preserve the conformational spatial arrangement of proteins in their native folded state, ensuring the biological activity of the protein.