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Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: human

Marques : Biosensis
Ratings: Ecrire un avis

Total Evaluation: 0
Moyenne des Notes : sur 5

BEK-2222-1P BEK-2222-2P
BSENBEK-2222-1PEA 398 EUR
BSENBEK-2222-1P BSENBEK-2222-2P
Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: human
Tests Tests ELISA

The Biosensis GDNF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed.


This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. A GDNF positive control (QC sample) is provided to assure consistent assay performance.


This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF


Guinea pig GDNF has been quantified in serum using the Human GDNF RapidTM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as 'human GDNF equivalents'.


This kit has not been tested for other applications.


Please refer to the kit protocol for specific use instructions for each substrate application.


Informations de livraison: Each kit contains 1 or 2×96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed protocols.


Attention: For research use only. Not for diagnostic or clinical purposes.

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SPÉCIFICATIONS

Environmentally Preferable
Cible GDNF
Primary antibody reactivity Human
Type de test Sandwich
Hôte
Sensibilité Typical limit of detection (LOD) for rhGDNF is <8 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10).
Portée de détection 7,8 – 500 pg/ml
Réactivité croisée No cross-reactivity was observed for the following proteins tested at 25 ng/mL: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Specificity Human. The antibodies used in this kit detect mouse and rat GDNF.

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