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VWR®, Taq DNA polymerase glycerol free

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733-1817EA 210 EUR
733-1817 733-1999 733-2038 733-2406 733-3843
VWR®, Taq DNA polymerase glycerol free
Réactifs pour acides nucléiques End-point PCR Enzymes and Kits

VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.


  • Most suitable choice for routine applications where high fidelity is not required
  • High performance, thermostable DNA polymerase
  • Optimal for TA cloning


VWR® Taq DNA Polymerase Glycerol Free has both a 5'→3' DNA polymerase and a 5'→3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). VWR® Taq DNA Polymerase Glycerol Free leaves an A' overhang, which makes the enzyme ideal for TA cloning.


10X Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween-20®


10X Extra Buffer: 100 mM Tris-HCL pH 8,3, 500 mM KCl, 15 mM MgCl₂, 1% Triton X-100


10X Mg-Free Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 1% Tween-20®


10X Mg-Free Extra Buffer: Tris-HCL pH 8,3, KCl, 1% Triton X-100

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SPÉCIFICATIONS

Désignation Taq DNA Polymerase Glycerol Free 5U/µl 1.000 units Taq DNA Polymerase Glycerol Free 5U/µl 5.000 units Taq DNA Polymerase Glycerol Free 5U/µl 2.000.000 units Taq DNA Polymerase Glycerol Free 5U/µl 100.000 units Taq DNA Polymerase Glycerol Free 5U/µl 375.000 units
Environmentally Preferable
Format Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer
Caractéristiques Glycerol free Glycerol free Glycerol free Glycerol free Glycerol free
Démarrage à chaud No No No No No
Concentration 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl
Systèmes 1000 5000 2000000 100000 375000
Taille 2 x 0.1 ml + 6 x 1.5 ml 10 x 0.1 ml Bulk 20 x 1 ml 1 x 75 ml
Composition du tampon 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 25 mM MgCl2 No buffer No buffer No buffer
Haute fidélité Non Non Non Non Non
Taille de l'amplicon ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard Standard
Activité exonucléase 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A 3'A
Conditions de stockage – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Application Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification Freeze-drying,Standard amplification

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